cd4-phycoerythrin (pe) cy7 sk3 Search Results


hla-drb1 antibody  (NSJ Bioreagents)
99
NSJ Bioreagents hla-drb1 antibody
Hla Drb1 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7-conjugated cd4 sk3  (Becton Dickinson)
90
Becton Dickinson pe-cy7-conjugated cd4 sk3
Pe Cy7 Conjugated Cd4 Sk3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4-phycoerythrin (pe) cy7 sk3  (Becton Dickinson)
90
Becton Dickinson cd4-phycoerythrin (pe) cy7 sk3
Cd4 Phycoerythrin (Pe) Cy7 Sk3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4-phycoerythrin (pe) cy7 sk3/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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cd4 phycoerythrin-cyanin 7 (pe-cy7) sk3  (Becton Dickinson)
90
Becton Dickinson cd4 phycoerythrin-cyanin 7 (pe-cy7) sk3
Cd4 Phycoerythrin Cyanin 7 (Pe Cy7) Sk3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4 phycoerythrin-cyanin 7 (pe-cy7) sk3/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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cd4: r-phycoerythrin (pe)–cyanine dye cy7 sk3  (Becton Dickinson)
90
Becton Dickinson cd4: r-phycoerythrin (pe)–cyanine dye cy7 sk3
Cd4: R Phycoerythrin (Pe)–Cyanine Dye Cy7 Sk3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4: r-phycoerythrin (pe)–cyanine dye cy7 sk3/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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mouse antihuman cd4 pe-cy 7 (clone# sk3, cat# 348789)  (Becton Dickinson)
90
Becton Dickinson mouse antihuman cd4 pe-cy 7 (clone# sk3, cat# 348789)
Mouse Antihuman Cd4 Pe Cy 7 (Clone# Sk3, Cat# 348789), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antihuman cd4 pe-cy 7 (clone# sk3, cat# 348789)/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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phycoerythrin-cyanine 7-conjugated anti-cd4  (Becton Dickinson)
90
Becton Dickinson phycoerythrin-cyanine 7-conjugated anti-cd4
Phycoerythrin Cyanine 7 Conjugated Anti Cd4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phycoerythrin-cyanine 7-conjugated anti-cd4/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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multitesttm 6-color tbnk  (Becton Dickinson)
90
Becton Dickinson multitesttm 6-color tbnk
<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Multitesttm 6 Color Tbnk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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phycoerythrin-cyanine7 (pe-cy7)-conjugated anti-cd4 [clone sk3]  (Thermo Fisher)
90
Thermo Fisher phycoerythrin-cyanine7 (pe-cy7)-conjugated anti-cd4 [clone sk3]
<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Phycoerythrin Cyanine7 (Pe Cy7) Conjugated Anti Cd4 [Clone Sk3], supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phycoerythrin-cyanine7 (pe-cy7)-conjugated anti-cd4 [clone sk3]/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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pe-cy™7  (Becton Dickinson)
90
Becton Dickinson pe-cy™7
<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Pe Cy™7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-cy™7/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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vα24 phycoerythrin (pe) (clone c15)  (Immunotec inc)
90
Immunotec inc vα24 phycoerythrin (pe) (clone c15)
Demographic, clinical and laboratory characteristics of control and CVID patient groups.
Vα24 Phycoerythrin (Pe) (Clone C15), supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vα24 phycoerythrin (pe) (clone c15)/product/Immunotec inc
Average 90 stars, based on 1 article reviews
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cd3 epsilon antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 epsilon antibody
Demographic, clinical and laboratory characteristics of control and CVID patient groups.
Cd3 Epsilon Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


T-, B- and NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: T-, B- and NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques:

ROC analysis of T-lymphocyte subset absolute counts for in-hospital mortality and disease severity of COVID-19 patients. Receiver operating characteristic (ROC) curves are represented for total CD3+ and four subsets of T lymphocyte cells. Cutoff values were identified with the Youden’s index. ( A ) ROC analysis showing the performance of baseline absolute counts of total T lymphocyte and their subsets in distinguishing fatal cases from survivors. Cutoff values for: CD3+: 524 cells/µl, sensitivity 67,65%, specificity: 78.57%, AUC: 0.7850, p < 0.0001; CD3 + CD4+: < 369 cells/µl, sensitivity 76.47%, specificity: 75.40%, AUC: 0.7697, p < 0.0001; CD3 + CD8+: < 194 cells/µl, sensitivity 73.53%, specificity: 70.63%, AUC: 0.7800, p < 0.0001; CD3 + CD4 + CD8 + DP: < 6.5 cells/µl, sensitivity 73.53%, specificity: 73.02%, AUC: 0.7715, p < 0.0001; CD3 + CD4 − CD8 − DN: < 21.5 cells/µl, sensitivity 76.47%, specificity: 68.25%, AUC: 0.7674, p < 0.0001. ( B ) ROC analysis showing the performance of baseline absolute counts of total T lymphocytes and their subsets in distinguishing severe (NRM, NIV and OTI) from nonsevere (AA and VMK) cases. Cutoff value for: CD3+ : < 733 cells/µl, sensitivity 69.44%, specificity: 73.86%, AUC: 0.7295, p < 0.0001; CD3 + CD4+ : < 426 cells/µl, sensitivity 62.50%, specificity: 72.73%, AUC: 0.6862, p < 0.0001; CD3 + CD8+ : < 262 cells/µl, sensitivity 73.61%, specificity: 64.77%, AUC: 0.7483, p < 0.0001; CD3 + CD4 + CD8 + DP: < 4.5 cells/µl, sensitivity 41.67%, specificity: 90.91%, AUC: 0.7148, p < 0.0001; CD3 + CD4 − CD8 − DN: < 18.5 cells/µl, sensitivity 56.94%, specificity: 84.09%, AUC: 0.7319, p < 0.0001. AUC area under the curve, AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: ROC analysis of T-lymphocyte subset absolute counts for in-hospital mortality and disease severity of COVID-19 patients. Receiver operating characteristic (ROC) curves are represented for total CD3+ and four subsets of T lymphocyte cells. Cutoff values were identified with the Youden’s index. ( A ) ROC analysis showing the performance of baseline absolute counts of total T lymphocyte and their subsets in distinguishing fatal cases from survivors. Cutoff values for: CD3+: 524 cells/µl, sensitivity 67,65%, specificity: 78.57%, AUC: 0.7850, p < 0.0001; CD3 + CD4+: < 369 cells/µl, sensitivity 76.47%, specificity: 75.40%, AUC: 0.7697, p < 0.0001; CD3 + CD8+: < 194 cells/µl, sensitivity 73.53%, specificity: 70.63%, AUC: 0.7800, p < 0.0001; CD3 + CD4 + CD8 + DP: < 6.5 cells/µl, sensitivity 73.53%, specificity: 73.02%, AUC: 0.7715, p < 0.0001; CD3 + CD4 − CD8 − DN: < 21.5 cells/µl, sensitivity 76.47%, specificity: 68.25%, AUC: 0.7674, p < 0.0001. ( B ) ROC analysis showing the performance of baseline absolute counts of total T lymphocytes and their subsets in distinguishing severe (NRM, NIV and OTI) from nonsevere (AA and VMK) cases. Cutoff value for: CD3+ : < 733 cells/µl, sensitivity 69.44%, specificity: 73.86%, AUC: 0.7295, p < 0.0001; CD3 + CD4+ : < 426 cells/µl, sensitivity 62.50%, specificity: 72.73%, AUC: 0.6862, p < 0.0001; CD3 + CD8+ : < 262 cells/µl, sensitivity 73.61%, specificity: 64.77%, AUC: 0.7483, p < 0.0001; CD3 + CD4 + CD8 + DP: < 4.5 cells/µl, sensitivity 41.67%, specificity: 90.91%, AUC: 0.7148, p < 0.0001; CD3 + CD4 − CD8 − DN: < 18.5 cells/µl, sensitivity 56.94%, specificity: 84.09%, AUC: 0.7319, p < 0.0001. AUC area under the curve, AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques:

Lymphocyte subset absolute counts in the five groups of patients stratified according to maximal oxygen supply/ventilation support. Absolute counts of total T-lymphocytes (CD3+, ( A ) and related subsets (CD3 + CD4+, ( B ) CD3 + CD8+, ( C ) CD3 + CD4 + CD8 + DP, ( D ) CD3 + CD4 − CD8 − DN, ( E ) CD4/CD8 ratio ( F ) B-lymphocytes (CD19+, ( G ) and NK-cells (CD3 neg CD16 + CD56+, ( H ) in the study population after stratification according to maximal oxygen supply/ventilation support required during hospitalization. Differences were analyzed using the Kruskal–Wallis and Dunn’s multiple comparison tests; the AA group was used as a reference group for multiple comparisons. Patients were further grouped into nonsevere (AA and VMK, blue dashed line box) and severe (NRM, NIV and OTI, red dashed line box); differences were analyzed using the Mann–Whitney test. A two-sided p value of < 0.05 was considered statistically significant. AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation. *0.01 < p < 0.5; **0.001 < p < 0.01; ***0.0001 < p < 0.00001; ****p < 0.0001.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Lymphocyte subset absolute counts in the five groups of patients stratified according to maximal oxygen supply/ventilation support. Absolute counts of total T-lymphocytes (CD3+, ( A ) and related subsets (CD3 + CD4+, ( B ) CD3 + CD8+, ( C ) CD3 + CD4 + CD8 + DP, ( D ) CD3 + CD4 − CD8 − DN, ( E ) CD4/CD8 ratio ( F ) B-lymphocytes (CD19+, ( G ) and NK-cells (CD3 neg CD16 + CD56+, ( H ) in the study population after stratification according to maximal oxygen supply/ventilation support required during hospitalization. Differences were analyzed using the Kruskal–Wallis and Dunn’s multiple comparison tests; the AA group was used as a reference group for multiple comparisons. Patients were further grouped into nonsevere (AA and VMK, blue dashed line box) and severe (NRM, NIV and OTI, red dashed line box); differences were analyzed using the Mann–Whitney test. A two-sided p value of < 0.05 was considered statistically significant. AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation. *0.01 < p < 0.5; **0.001 < p < 0.01; ***0.0001 < p < 0.00001; ****p < 0.0001.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: MANN-WHITNEY

Univariable and multivariable logistic regression analysis for in-hospital mortality-related risks in patients with SARS-CoV-2 infection.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Univariable and multivariable logistic regression analysis for in-hospital mortality-related risks in patients with SARS-CoV-2 infection.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Infection

Univariable and multivariable logistic regression analysis for severity-related risks in patients with SARS-CoV-2 infection.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Univariable and multivariable logistic regression analysis for severity-related risks in patients with SARS-CoV-2 infection.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Infection

Correlation between lymphocyte subpopulation absolute counts and inflammation markers. Absolute counts of total T-lymphocytes and related subsets (CD3 + CD4+, CD3 + CD8+, CD3 + CD4 + CD8 + DP, CD3 + CD4 − CD8 − DN), B-lymphocytes and NK-cells (CD16 + CD56+) were correlated with IL-6 (column A), CRP serum concentrations (column B) and d -dimer plasma concentration (column C). Correlation was assessed with the Spearman’s test; Spearman r (only if statistically significant) and p are reported in the boxes; a two-sided p value of < 0.05 was considered statistically significant. ns not statistically significant, IL-6 interleukin-6, CRP C-reactive protein.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Correlation between lymphocyte subpopulation absolute counts and inflammation markers. Absolute counts of total T-lymphocytes and related subsets (CD3 + CD4+, CD3 + CD8+, CD3 + CD4 + CD8 + DP, CD3 + CD4 − CD8 − DN), B-lymphocytes and NK-cells (CD16 + CD56+) were correlated with IL-6 (column A), CRP serum concentrations (column B) and d -dimer plasma concentration (column C). Correlation was assessed with the Spearman’s test; Spearman r (only if statistically significant) and p are reported in the boxes; a two-sided p value of < 0.05 was considered statistically significant. ns not statistically significant, IL-6 interleukin-6, CRP C-reactive protein.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Concentration Assay

T-, B- and NK-lymphocyte subpopulation absolute counts at baseline after patient stratification according to maximal oxygen supply/ventilation support.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: T-, B- and NK-lymphocyte subpopulation absolute counts at baseline after patient stratification according to maximal oxygen supply/ventilation support.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques:

Demographic, clinical and laboratory characteristics of control and CVID patient groups.

Journal: PLoS ONE

Article Title: Skewed Distribution of Circulating Activated Natural Killer T (NKT) Cells in Patients with Common Variable Immunodeficiency Disorders (CVID)

doi: 10.1371/journal.pone.0012652

Figure Lengend Snippet: Demographic, clinical and laboratory characteristics of control and CVID patient groups.

Article Snippet: The following monoclonal antibodies were used in the assays: CD3-peridin chlorophyll protein (PerCP) (clone SK7), CD8-allophycocyanin (APC) (clone SK1) and CD4-phycoerythrin–cyanine (PE-Cy7) (clone SK3), from BD Biosciences (San Jose, CA); CCR5-PE-Cy7 (clone 2D7/CCR5) and CD161-APC (clone DX12) from BD PharMingen (San Jose, CA); Vα24 phycoerythrin (PE) (clone C15), Vβ11-Fluorescein isothiocyanate (FITC) (clone C21) from Immunotech (BC); CXCR6-APC (clone 56811) and CD69-allophycocyanin cyanine-7 (APC-Cy7) (clone FN50).

Techniques: Control, Biomarker Discovery

(A) Representative flow cytometric analyses on PBMC, lymphocytes, CD3+ T cells and Vα24+Vβ11+ for NKT cells. (B) Fluorescence minus one (FMO) was used for gate strategy for CXCR6, CCR5 and CD69 in NKT cells. (C) Representative flow cytometric analyses on NKT cells in CVID patients. Comparisons among groups were carried out using the Mann-Whitney non-parametric test.

Journal: PLoS ONE

Article Title: Skewed Distribution of Circulating Activated Natural Killer T (NKT) Cells in Patients with Common Variable Immunodeficiency Disorders (CVID)

doi: 10.1371/journal.pone.0012652

Figure Lengend Snippet: (A) Representative flow cytometric analyses on PBMC, lymphocytes, CD3+ T cells and Vα24+Vβ11+ for NKT cells. (B) Fluorescence minus one (FMO) was used for gate strategy for CXCR6, CCR5 and CD69 in NKT cells. (C) Representative flow cytometric analyses on NKT cells in CVID patients. Comparisons among groups were carried out using the Mann-Whitney non-parametric test.

Article Snippet: The following monoclonal antibodies were used in the assays: CD3-peridin chlorophyll protein (PerCP) (clone SK7), CD8-allophycocyanin (APC) (clone SK1) and CD4-phycoerythrin–cyanine (PE-Cy7) (clone SK3), from BD Biosciences (San Jose, CA); CCR5-PE-Cy7 (clone 2D7/CCR5) and CD161-APC (clone DX12) from BD PharMingen (San Jose, CA); Vα24 phycoerythrin (PE) (clone C15), Vβ11-Fluorescein isothiocyanate (FITC) (clone C21) from Immunotech (BC); CXCR6-APC (clone 56811) and CD69-allophycocyanin cyanine-7 (APC-Cy7) (clone FN50).

Techniques: Fluorescence, MANN-WHITNEY

(A) Percentage of chemokine receptor CCR5 in NKT cells gate (Vα24+Vβ11) in representative healthy subject and CVID patient (p<0.0001). (B) Percentage of chemokine receptors CXCR6, CCR5 and CD69 marker in NKT cells (p<0.001). (C) Percentage of chemokine receptor CCR5 and CD69 marker in NKT cells (p<0.001). Comparisons among groups were carried out using the Mann-Whitney non-parametric test.

Journal: PLoS ONE

Article Title: Skewed Distribution of Circulating Activated Natural Killer T (NKT) Cells in Patients with Common Variable Immunodeficiency Disorders (CVID)

doi: 10.1371/journal.pone.0012652

Figure Lengend Snippet: (A) Percentage of chemokine receptor CCR5 in NKT cells gate (Vα24+Vβ11) in representative healthy subject and CVID patient (p<0.0001). (B) Percentage of chemokine receptors CXCR6, CCR5 and CD69 marker in NKT cells (p<0.001). (C) Percentage of chemokine receptor CCR5 and CD69 marker in NKT cells (p<0.001). Comparisons among groups were carried out using the Mann-Whitney non-parametric test.

Article Snippet: The following monoclonal antibodies were used in the assays: CD3-peridin chlorophyll protein (PerCP) (clone SK7), CD8-allophycocyanin (APC) (clone SK1) and CD4-phycoerythrin–cyanine (PE-Cy7) (clone SK3), from BD Biosciences (San Jose, CA); CCR5-PE-Cy7 (clone 2D7/CCR5) and CD161-APC (clone DX12) from BD PharMingen (San Jose, CA); Vα24 phycoerythrin (PE) (clone C15), Vβ11-Fluorescein isothiocyanate (FITC) (clone C21) from Immunotech (BC); CXCR6-APC (clone 56811) and CD69-allophycocyanin cyanine-7 (APC-Cy7) (clone FN50).

Techniques: Marker, MANN-WHITNEY